The Physics Of Gas Chromatography Essay - 1814 Words

In gas chromatography, the components of the sample, (which can be called solutes or analytes), separate between two phases – a stationary phase that has a large surface area and a gas phase that permeates through the stationary surface (McNair Miller, 1998). The technique begins when a few microliters of liquid sample are injected into a port and it becomes vaporized to a gas in the chamber. This starts the mobile phase (also known as the carrier gas). Then, an inert carrier gas is fed into the injection port chamber containing the sample in gas form, and pushes the gas molecules through (Hübschmann, 2001). It is important that the carrier gas is inert so that it doesn’t react with the different molecules of the sample we are trying to separate. Once in this first chamber, it begins to heat and travel through a long coiled tube called a gas chromatography (GC) column. The next stop on the coiled column is the detector, which identifies the amount of each compound is present in our sample. There are a series of factors that affect the rate at which compounds reach the detector which will be described in the following sections, but for now it is important to note that the lower boiling point and the less molecular weight, the faster the sample components will separate and migrate to the detector (McNair Miller, 1998). Any individual peak produced can widen or enlarge as the chromatographic analysis ensues (McNair Miller, 1998). In addition, the area of each peak isShow MoreRelatedInvestigating The Substances Of Water Chromatography1350 Words   |  6 Pagesmight be present in a sample. Chromatography is one of the first tools used in such situations. In this technique, many types of mixtures can be separated into the component pure substances; by comparison to a standard sample, each component substance can also be tentatively identified. Many v arieties of chromatography exist, each one designed to separate specific types of mixtures. The common feature of each type of chromatography is that a mobile phase (a liquid or gas) is pushed through a stationaryRead MoreMass Spectrometry ( Working Principle )1050 Words   |  5 Pagessample under investigation, it then separates them according to their specific mass-to-charge ratio (m/z), and then records the relative abundance of each ion type. The first step in the mass spectrometric analysis of compounds is the production of gas phase ions of the compound, basically by electron ionization. This molecular ion undergoes fragmentation. Each primary product ion derived from the molecular ion, in turn, undergoes fragmentation, and so on. The ions are separated in the mass spectrometerRead MoreThin Layer Chromatography And Column Chromatography1774 Words   |  8 Pages Thin Layer Chromatography and Column Chromatography By Maggi Shelton Under the Supervision of Dr. Mills Chavonda The Department of Chemistry and Physics Milledgeville, GA 31061 â€Æ' Abstract: Thin layer chromatography and column chromatography are two different methods that allow for the separation of two miscible solvents. Through column chromatography, a mixture of nonpolar fluorine and polar fluorenone was successfully separated. Thin layer chromatography was then used in order to determineRead MoreEssay on Forensic Science1603 Words   |  7 Pagesdegree and an apprenticeship in a medical examiners office. In the field of forensic science, there are many subspecialties. 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School friends later recalled that she was â€Å"best in science, best at maths, best in everything.† http://www.livescience.com/39804-rosalind-franklin.html Rosalind’’s father wanted her to go into the line of social work, but at age fifteenRead MoreAnalytical Chemistri Intro1796 Words   |  8 Pagesaquatic organisms, plants and animals. 17 Geology Geophysics Geochemistry Palaeontology Paleobiology Biology Botany Genetics Microbiology Molecular biology Zoology Chemistry Biochemistry Inorganic chem. Organic chem. Physical chem. Physics Astrophysics Biophysics Environmental sciences Ecology Meteorology Oceanography Analytical chemistry Medicine Agriculture Agronomy Animal science Crop science Food science Soil science Horticulture Engineering Civil Chemical ElectricalRead MoreSynthesis Of Triphenylamine Based Dye And Fabrication Of Dssc Device3205 Words   |  13 Pages Figure 1: Principle of operation of a DSSC. 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The radionuclide introduced into the body is often chemically bound to aRead MoreExample Of Data Processing8068 Words   |  33 Pagesby electronic noses (Schiffman et al., 2000). The elimination of this weakness may allow electronic noses to become a cheaper, quicker, and more mobile means of mold detection than the traditional methods of polymerase chain reaction (PCR), gas chromatography-ma ss spectrometry (GC-MS), and mycological analysis (Paolesse et al., 2006). The use of electronic noses may prevent numerous cases of SBS caused by the growth of fungal contaminants, including those of Alternaria, Aspergillus, Chaetomium,Read MorePharmaeutical Industry Training Report with Deep Description of Different Area in Ibn Sina Pharmaceutical Industry Ltd.14426 Words   |  58 Pages | |Potentiometer |Potentiometer | |HPLC( High Performance Liquid Chromatography) |Sonicator | |Atomic Absorption Spectrophotometer |Vacuum Oven | |UV- Spectrophotometer